A coalition of 16 attorneys general filed a multistate amicus brief Friday in support of the Biden administration’s newly adopted Title IX rules to protect trangender students, which are facing multiple legal challenges.

Several lawsuits have been filed in federal court following the U.S. Department of Education’s release of the final version of Title IX regulations on April 19. Litigation has been initiated in Alabama, Kentucky, Louisiana and Texas federal courts, collectively challenging the inclusion of gender identity in the definition of discrimination based on sex.